Who manual for the standardized investigation, diagnosis and management of the infertile male. The integrity of the paternal genome is of crucial im portance to reach our goal of a healthy newborn. Although sperm with fragmented dna can penetrate the oocyte henkel et al, 2004, embryo development would be greatly compromised, since the dna repair capacity of the oocyte is very limited twigg et al. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. O dna como material genetico, replicacao, transcricao. Dna damage expressed as dna fragmentation index dfi and percentage of the dna undamaged spermatozoa expressed as big halo. Infertility affects almost 20% of couples in reproductive age and the. Fragmentacion del adn espermatico e infertilidad masculina.
Dna fragmentation in human fibroblasts under extremely low. To determine whether the use of magnetic activated cell sorting macs as a sperm selection technique improves art success rates in couples undergoing assisted reproduction treatment. Fragmentacion del adn espermatico en verracos articulos. Dna fragmentation is a factor independent from traditional semen tests. The integrity of the paternal genome is of paramount importance in the initiation and maintenance of a viable pregnancy in vivo and in vitro. Sperm dna integrity is a sperm functional parameter of male fertility evaluation.
The presence in the embryonic genome of dna strand breaks andor modifications in dna nucleotides originating in the paternal genome, which have not been repaired by the oocyte after fertilization, is not compatible with normal embryo and fetal development. Dna fragmentation, sperm dna fragmentation, fragmentation of human sperm dna and dna fragmentation tests. Estructura y funcion del material genetico by ramiro deiver. Two parameters of sperm dna integrity were observed.
Fragmentacion del adn espermatico y fertilizacion youtube. Total antioxidant capacity, although not compromised. We found a greater susceptibility to damage generated in the laboratory procedures in the samples with lower quality. Current techniques are far from perfect in terms of predictability, selection and results. Dna fragmentation is the oxidative stresses which together to the. Fragmentacion del adn espermatico en espermogramas normales. Sperm selection using magnetic activated cell sorting. Singlestranded dna damage shows its major effect at the pronuclei stage, but no increase of delay is shown at other stages. Increased aneuploidy rate in sperm with fragmented dna as. Dna breaks as a result of apoptotic dna degradation or persistence of unrepaired dna nicks should correspond to a massive dna damage level. Alternatively, doublestranded dna damage causes a delay at the extrusion of the second polar body, but the embryo development delay shows a progressive increase as embryo development proceeds.
Jan 05, 2010 dna fragmentation in human fibroblasts under extremely low frequency electromagnetic field exposure. Cb2 receptors are less frequent and found predominately in immune system cells munro et al. Halosperm test was used for sperm dna integrity determination. Fragmentation of sperm dna using the tunel method actas. Doublestranded sperm dna damage is a cause of delay in. The sample of choice for evaluating dna fragmentation will depend on whether the clinician is treating a natural or assisted fertilization. Neuroprotection by the endogenous cannabinoid anandamide and arvanil against in vivo excitotoxicity in the rat. For completing study of male factor is necessary analyze other parameters like the dna. Sperm dna fragmentation and its relationship to fertility is a topic of great interest, not only in humans but in all species, given the implicit role of the dna molecule in producing a successful.